Composite

Part:BBa_K2139008

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-14)


G12 Expression Cassette

This part encompass the coding region for an endo-beta-1,4-glucanase known as G12. G12 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases and beta-1,4-glucosidases for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is ABK52392.1 (amino acid) and a PDB code of 1H0B_A (homolog).

Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 864


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