Part:BBa_K2138001

RNA thermometer and RBS (39C)

The RNA thermometer portion of the part is designed to linearize and allow translation of a target protein at thirty-nine degrees Celsius. The ribosome binding site was incorporated into the design and selected following the Anderson ribosome binding site format for optimal protein expression and DNA design. The entire part is able to be restricted from the pSB1C3 backbone and ligated with any type of reporter protein to measure temperature regulated expression.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 1
  Illegal suffix found in sequence at 193
  Illegal EcoRI site found at 2262
  Illegal XbaI site found at 2277
  Illegal SpeI site found at 215
  Illegal PstI site found at 229
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 2262
  Illegal NheI site found at 30
  Illegal NheI site found at 53
  Illegal SpeI site found at 194
  Illegal SpeI site found at 215
  Illegal PstI site found at 208
  Illegal PstI site found at 229
  Illegal NotI site found at 7
  Illegal NotI site found at 201
  Illegal NotI site found at 222
  Illegal NotI site found at 2268
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 2262
  Illegal XhoI site found at 1246
  Illegal XhoI site found at 2138
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1
  Illegal suffix found in sequence at 194
  Illegal EcoRI site found at 2262
  Illegal XbaI site found at 2277
  Illegal SpeI site found at 215
  Illegal PstI site found at 229
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1
  Illegal EcoRI site found at 2262
  Illegal XbaI site found at 16
  Illegal XbaI site found at 2277
  Illegal SpeI site found at 194
  Illegal SpeI site found at 215
  Illegal PstI site found at 208
  Illegal PstI site found at 229
  
• 1000
  COMPATIBLE WITH RFC[1000]