RBS
Part:BBa_K2138000
Designed by: Austin Nykiel Group: iGEM16_UMass-Dartmouth (2016-10-19)
RNA thermometer and RBS (40C)
The RNA thermometer portion of the part is designed to linearize and allow translation of a target protein at forty degrees Celsius. The ribosome binding site was incorporated into the design and selected following the Anderson ribosome binding site format for optimal protein expression and DNA design. The entire part is able to be restricted from the pSB1C3 backbone and ligated with any type of reporter protein to measure temperature regulated expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 118
Illegal EcoRI site found at 2166
Illegal XbaI site found at 2181 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2166
Illegal NheI site found at 30
Illegal NheI site found at 53
Illegal SpeI site found at 119
Illegal PstI site found at 133
Illegal NotI site found at 7
Illegal NotI site found at 126
Illegal NotI site found at 2172 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2166
Illegal XhoI site found at 1150
Illegal XhoI site found at 2042 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 119
Illegal EcoRI site found at 2166
Illegal XbaI site found at 2181 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 2166
Illegal XbaI site found at 16
Illegal XbaI site found at 2181
Illegal SpeI site found at 119
Illegal PstI site found at 133 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |