Coding

Part:BBa_K2130010

Designed by: Ooi Kean Hean   Group: iGEM16_NTU-Singapore   (2016-10-19)


∆REC1-3 SP-dCas9

dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner. For this part, we have deleted the HNH domain of the SP-dCas9 and tested it's binding capability by assessing the strength in transcriptional activation via a VP64-p65-Rta.

T--NTU-Singapore--rec1.png

T--NTU-Singapore--rec1del.png

T--NTU-Singapore--rec1delresult.png

T--NTU-Singapore--rec1micro.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None