Reporter

Part:BBa_K2130004

Designed by: Ooi Kean Hean   Group: iGEM16_NTU-Singapore   (2016-10-19)


Report plasmid with toxin ccdB gene and inducible BAD promoter

The report plasmid encodes the toxin ccdB gene under the inducible BAD promoter. The plasmid contains a Ampicillin gene which the gRNA is designed to target at.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1437
    Illegal BsaI site found at 1756
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None