Reporter

Part:BBa_K2130003

Designed by: Ooi Kean Hean   Group: iGEM16_NTU-Singapore   (2016-10-19)


ZsGreen(GFP) reporter with 2X dCas9-inducible promoter

The reporter is regulated by a dCas9-VPR inducible promoter which has 2 Streptococcus pyogenes dCas9 or SAdCas9 binding sites. The reporter is usable in mammalian cells only.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 430
    Illegal XhoI site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 708
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None