Coding

Part:BBa_K2128010

Designed by: David Sanderson   Group: iGEM16_Genspace   (2016-10-18)


RFP Optimzed for C. Elegans

This is a RFP encoding ORF, it was taken from BBa_E1010, a "highly engineered mutant of red fluorescent protein from Discosoma striata". It was codon optimized for C. Elegans by IDT's Codon Optimization Tool.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 349
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 349
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 349
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 349
    Illegal AgeI site found at 399
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None