Composite

Part:BBa_K2128007

Designed by: David Sanderson   Group: iGEM16_Genspace   (2016-08-13)


GFP with H. Dujardini ACTN Promoter, RBS and Terminator

This part encodes for a Green Fluorescent Protein in H. Dujardini and potentially other Tardigrade species.

The GFP in this part was codon optimized Optimized for C. Elegans by IDT's Codon Optimization Tool.

The promoter, RBS and Terminator were taken from the H. Dujardini ACTN gene. It is a constitutive promoter that is on at all stages of development.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1218
    Illegal BamHI site found at 997
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58
    Illegal SapI site found at 280


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Categories
Parameters
None