Coding

Part:BBa_K2128003

Designed by: David Sanderson   Group: iGEM16_Genspace   (2016-08-13)


RVLEAM

This part is a member is a class of intrinsically disordered proteins called the Late Embryonic Abundant (LEA) proteins that become ordered when desiccated, thereby stabilizing DNA and protein cell components. This tardigrade unique protein is found in the mitochondria of tardigrade cells. When cloned into humans, they confer protection against osmotic stress as shown in the paper "Novel Mitochondria-Targeted Heat-Soluble Proteins Identified in the Anhydrobiotic Tardigrade Improve Osmotic Tolerance of Human Cells" by Sae Tanaka et al. 2015 Feb 12.

It was taken from the genome of Ramazzottius varieornatus and optimized by the IDT Codon Optimization Tool for E. Coli K12.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 124
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None