Protein_Domain

Part:BBa_K2124003

Designed by: Brian Dempsey   Group: iGEM16_Lethbridge_HS   (2016-10-13)


Venom Serine Protease from Cerastes Cerastes - Thrombin analog - DsbC Disulfide shuffling

This Serine protease is a component of Cerastes Cerastes (Desert Horned Viper) venom. It recognizes Thrombin cut sites in fibrinogen and upon cleavage converts the fibrinogen to fibrin monomers. The fibrin monomers can then link and begin clot formation in mammals. This part is polycistronic and also contains DsbC which is secreted to the periplasmic space. DsbC shuffles the Serine Protease disulfide bonds for proper folding and protein activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None