Regulatory
Part:BBa_K2123106
Designed by: Maria Clara Tavares Astolfi Group: iGEM16_UFAM-UEA_Brazil (2016-10-13)
Universal promoter (Ptac + PJK26) + mer operator
New promoters regulated by MerR library
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 89
Illegal EcoRI site found at 2
Illegal XbaI site found at 17 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2
Illegal SpeI site found at 90
Illegal PstI site found at 104
Illegal NotI site found at 8
Illegal NotI site found at 97 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2
Illegal BglII site found at 20 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2
Illegal suffix found in sequence at 90 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2
Illegal XbaI site found at 17
Illegal SpeI site found at 90
Illegal PstI site found at 104 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |