Device

Part:BBa_K2122200

Designed by: Sannia Farrukh   Group: iGEM16_NYU-AD   (2016-10-13)


His-tagged Shiga-Like Subunit B toxin

Shiga-like toxin subunit B encoding sequence, his-tagged on both ends, under the control of an arabinose inducible promoter and RBS. Followed by a double promoter. It is used to code for Shiga-like toxin subunit B that binds to Gb3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1910
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2193


[edit]
Categories
Parameters
None