Device

Part:BBa_K2122100

Designed by: Sannia Farrukh   Group: iGEM16_NYU-AD   (2016-09-29)


Device expressing the Gb3 Synthase enzyme under control of a constitutive promoter

Gb3 synthase encoding sequence under the control of a constitutive promoter and RBS. Followed by a double promoter. It is used to code for Gb3 that binds to Shiga-like toxin subunit B.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 735
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1018


[edit]
Categories
//chassis/prokaryote/ecoli
//promoter/anderson
//rbs/prokaryote/constitutive/community
//terminator/double
Parameters
None