Reporter

Part:BBa_K2121013

Designed by: Leslie Wang   Group: iGEM16_Yale   (2016-10-21)


AStr_Citrine_T1

Anderson promoter, citrine fluorescence reporter, and T1 terminator cassette for rhizobia promoter characterization with LIC sites flanking the protein CDS.

Promoter sequence from BioBrick J23100, Anderson promoter library.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1914
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1881
    Illegal BsaI.rc site found at 55


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Categories
Parameters
None