Coding

Part:BBa_K2121004

Designed by: Jinny van Doorn   Group: iGEM16_Yale   (2016-10-20)


Lambda_Beta_F7X3D1 (red_rhizo-MAGE)

Coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (S. meliloti and CIAT 899). Identified with BLASTp from homology to the lambda coliphage beta recombinase. Derived from phage that selectively infects rhizobia spp (Sinorhizobium meliloti strain SM11)

Within Yale iGEM 2016, known as gBlock 15, amplified for cloning with primers priG 461, 462

Unitprot KB F7X3D1_SINMM, E value 1.50E-36

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 511
    Illegal NgoMIV site found at 536
    Illegal NgoMIV site found at 914
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None