Composite

Part:BBa_K212002

Designed by: Elias Scheer   Group: iGEM09_Brown   (2009-10-19)


OmpC-RFP (promoter-reporter transduction readout)

This construct is a reporter, which is activated by the EnvZ kinase domain of Taz. EnvZ phosphorylates transcription factor OmpR, which in turn binds to the OmpC promoter. This cassette contains the promoter region of OmpC (BBa_R0082), ligated in front of red fluorescence protein (RFP) gene (BBa_E1010). The ribosome binding site is . There is also a double terminator sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 689
    Illegal AgeI site found at 801
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None