Regulatory

Part:BBa_K2119131

Designed by: Macarena Natalia Ramos Briones   Group: iGEM16_Saint_Rose_School_B   (2016-10-14)


Cellobiose response regulator (ClbR) is a Zn(II)2Cys6 transcriptional activator.

ClbR controls the expression of cellulase-related genes in response to Avicel and cellobiose in Aspergillus aculeatus. Cellobiose bound to ClbR and active Cbh1. through this transcriptional activator detecting cellulose will allow the gene expression, the Lip and Lac, and that way degrade lignin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 669
    Illegal BamHI site found at 1701
    Illegal BamHI site found at 1831
    Illegal XhoI site found at 877
    Illegal XhoI site found at 1616
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 2272
    Illegal AgeI site found at 946
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2362
    Illegal SapI.rc site found at 428


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Categories
Parameters
None