Composite

Part:BBa_K2117011

Designed by: Isabella Loft   Group: iGEM16_DTU-Denmark   (2016-10-14)


ECFP under regulation of the Y. lipolytica native promoter TEF1

This part is a composite part consisting of the Y. lipolytica promoter TEF1 (BBa_K2117000) and a engineered cyan fluorescent protein (eCFP) derived from A. victoria GFP from the Biobrick BBa_E0020 made by the group Antiquity in 2004. It was further shown to be functional in E. coli by the British Columbia 2012 team.

The emission of CFP is seen at a wavelength of 439nm when excited at 476nm.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


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Categories
Parameters
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