Composite

Part:BBa_K2117010

Designed by: Isabella Loft   Group: iGEM16_DTU-Denmark   (2016-10-14)


eYFP expressed by the constitutive Y. lipolytica promoter TEF1

This part is a composite part consisting of the Y. lipolytica promoter TEF1 (BBa_K2117000) and an enhanced yellow fluorescent protein (eYFP) from the Biobrick BBa_E0030.

The enhanced yellow fluorescent protein is derived from an A. victoria GFP.

Excitation: 515/514nm Emission: 528/527nm

BBa_E0030 has been tested and characterized by both BIOSINT_Mexico iGEM 2014 and the British Columbia 2012 team.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


[edit]
Categories
Parameters
None