Composite

Part:BBa_K2117002

Designed by: Isabella Loft   Group: iGEM16_DTU-Denmark   (2016-10-14)


TEF1 promoter with proinsulin

This is a composite part encoding the translation elongation factor-1α (TEF1) promoter and the gene encoding the Human Proinsulin peptide.

The TEF1 promoter is a strong constitutive promoter. Native for the oleaginous yeast Yarrowia lipolytica. This promoter is often used for isolation of enzyme genes by expression cloning. However, the promoter is usually not recommended for heterologous production due to early expression of heterologous genes can be detrimental for cell growth.

The Human Proinsulin gene has been codon-optimized for the yeast Yarrowia lipolytica using a DTU-Denmark iGEM 2016 team designed codon-optimization tool that also considers illegal restriction sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


[edit]
Categories
Parameters
None