Coding

Part:BBa_K2114013

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-09)


GST_HA_G4S_CotB

N-terminal fusion of glutathione S-transferase to spore crust gene cotB by a flexible GGGGS linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1750
    Illegal BamHI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85
    Illegal SapI.rc site found at 1204


[edit]
Categories
Parameters
None