Composite

Part:BBa_K2113010

Designed by: SVCE_CHENNAI   Group: iGEM16_SVCE_CHENNAI   (2016-10-14)


RNA thermometer and AMP with glycine

This construct consists of RNA thermometer (BBa_K115001) for temperature regulated system under the control of a strong promoter (BBa_J23119).It is then followed by antimicrobial peptides with glycine (BBa_K2113005),Cj blue being used as a reporter protein (BBa_K592011).Double terminator(BBa_K823017)is used to terminate the transcription.This construct could be used to study the effect of antimicrobial peptides regulated by temperature sensitive system activated above 42 degree celcius.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None