Composite

Part:BBa_K2113003

Designed by: SVCE_CHENNAI   Group: iGEM16_SVCE_CHENNAI   (2016-10-13)


temperature sensitive system with RNA thermometer

The RNA thermometer (BBa_K115001) is expressed under the regulation of the part (BBa_J23119) which contains a strong promoter. Following the RNA thermometer the construct contains GFP (BBa_E0040) as the reporter which is followed by the double terminator (BBa_K823017).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 789


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Categories
Parameters
None