Composite

Part:BBa_K2113002

Designed by: SVCE_CHENNAI   Group: iGEM16_SVCE_CHENNAI   (2016-10-13)


temperature sensitive system with lambda pr promoter under cI regulation

It is a construct which contains a complete temperature sensitive system with lambda pr as a promoter (BBa_K608351) activated at 42 degree celcius with gfp (BBa_E0040) as its reporter to study the expression at various temperatures.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1618



RESULTS -SVCE_Chennai 2016

temperature sensitive system with lambda pr promoter under cI regulation We constructed the λpR promoter with GFP in order to study the expression at 37degree Celsius , 42 degrees Celsius and 47 degree celsius. The fluorescence was measured and plotted as a graph. It was found that expression was higher at 42 degrees Celsius. And size conformation was done and the band was found at 3600bp.


Plate 1.png

Plate 2.png


G 1.png

G 2.png

G 3.png

G 4.png

[edit]
Categories
Parameters
None