Part:BBa_K2112011
Fragment 1
T7 promoter is a strong promoter to launch the transcription procedure. Lac operator DNA sequence could bind a protein named LacI coded by a lacI DNA sequence which located in another plasmid. This process stop the transcription of the target gene (opdA). So in our study, we add a compound named IPTG to the culture medium. IPTG could bind LacI protein, so that the structure of LacI will be changed and could not bind to lac operator DNA sequence any more. And the target gene opdA could be transcribed and translated. His-tag sequence (fused in N-terminal of target protein OpdA) consisted of five to six histidines, which could bind to Nickel metal ions. So with a special resin chelated with nickel metal ions, we can purify the expressed OpdA protein easily.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal XbaI site found at 67 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal XbaI site found at 67 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal XbaI site found at 67 - 1000COMPATIBLE WITH RFC[1000]
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