Designed by: Haoran Ding Group: iGEM16_Tianjin (2016-10-12)
PETase site-directed mutant A240P
This is one of the site-directed mutant of the PETase gene, we change the 240th amino acid from A to P. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21INCOMPATIBLE WITH RFCIllegal BglII site found at 79
Illegal BglII site found at 289
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.