Coding

Part:BBa_K2110016

Designed by: Le He   Group: iGEM16_Tianjin   (2016-10-06)


The key gene for utilization of ethylene glycolin P.putida KT2440.

This gene controls the synthesis of isocitratelyase, which controls the crucial step to utilize ethylene glycol in the P. putidaKT2440. The Overexpression of aceA leads to an increasing of utilization of ethylene glycol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 34
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 271
    Illegal NgoMIV site found at 436
    Illegal NgoMIV site found at 565
    Illegal NgoMIV site found at 823
    Illegal NgoMIV site found at 1081
    Illegal AgeI site found at 1294
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1309


[edit]
Categories
Parameters
None