Coding
Part:BBa_K2110014
Designed by: Le He Group: iGEM16_Tianjin (2016-10-06)
MHETase enzyme after codon optimization for bacillus subtilis
This gene encodes the MHETase enzyme. This protein can bind on the PET surface and degrade MHET to TPA and EG, which have higher efficiency than other cutinase. This enzyme cooperates with PETase enzyme well in PET degradation. At first, this gene couldn’t fully express in bacillus subtilis. So we use codon optimization for bacillus subtilis for better expression and secretion.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 91
Illegal NgoMIV site found at 387
Illegal AgeI site found at 1086 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
chassis | bacillus subtilis |