Coding
Part:BBa_K2110013
Designed by: Le He Group: iGEM16_Tianjin (2016-10-06)
PETase enzyme after codon optimization for bacillus subtilis
This gene encodes thePETase enzyme. This protein can bind on thePET surface and degrade PET to MHET, which have higher efficiency than other cutinase. At first, this gene couldn’t fully express in bacillus subtilis. So we use codon optimizationfor bacillus subtilis for better expression and secretion.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 639
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
chassis | bacillus subtilis |