Composite
Part:BBa_K2109106
Designed by: Andy Hudson Group: iGEM16_Lethbridge (2016-10-14)
AraC-pBAD-RNAPa
This part encodes RNA polymerase under the control of the arabinose-inducible pBAD promoter. A multiple cloning site was introduced 3' of the RNA polymerase coding sequence in order to generate a C-terminal fusion to a target protein.
Overexpression of the RNA polymerase fusion protein upon arabionse induction was attempted. However, the RNA polymerase fusion protein has a size similar (if not indistinguishable from) to the endogenous RNA polymerase within E. coli. As there is no antibody specific for this specific fusion protein, a Western Blot could not be performed to confirm the levels of and therefore the overexpression of the RNA polymerase fusion protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2055
Illegal BamHI site found at 1144
Illegal XhoI site found at 2091 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1763
Illegal AgeI site found at 979 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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Categories
Parameters
None |