Part:BBa_K2109104
Fluorescent Bacterial-2-Hybrid Reporter Cassette
Fluorescent Bacterial-2-Hybrid Reporter Cassette.
This part contains mRFP1 coding sequence with a weak constitutive promoter and strong RBS with low overall mRFP1 expression. The mRFP1 expression is designed to increase in the presence of an interaction between a single antibody and a target antigen protein that are C-terminally fused to lambda cI (BBa K2109103) and RNA polymerase alpha subunit (BBa K2109106), respectively. This part also encodes mTagBFP under the control of a strong promoter and strong RBS, however on the opposite strand. The mRFP1 and mTagBFP genes are separated by a strong bi-directional intrinsic terminator to prevent cross-talk between the upstream and downstream genes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 130
Illegal NheI site found at 153
Illegal NheI site found at 1822 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 84
Illegal XhoI site found at 118 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |