Composite

Part:BBa_K2109104

Designed by: Andy Hudson   Group: iGEM16_Lethbridge   (2016-10-14)


Fluorescent Bacterial-2-Hybrid Reporter Cassette

Fluorescent Bacterial-2-Hybrid Reporter Cassette.

This part contains mRFP1 coding sequence with a weak constitutive promoter and strong RBS with low overall mRFP1 expression. The mRFP1 expression is designed to increase in the presence of an interaction between a single antibody and a target antigen protein that are C-terminally fused to lambda cI (BBa K2109103) and RNA polymerase alpha subunit (BBa K2109106), respectively. This part also encodes mTagBFP under the control of a strong promoter and strong RBS, however on the opposite strand. The mRFP1 and mTagBFP genes are separated by a strong bi-directional intrinsic terminator to prevent cross-talk between the upstream and downstream genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 130
    Illegal NheI site found at 153
    Illegal NheI site found at 1822
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 84
    Illegal XhoI site found at 118
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None