Composite

Part:BBa_K2109102

Designed by: Andy Hudson   Group: iGEM16_Lethbridge   (2016-10-14)


lambda cl-Nb (BH-GBP3)

This part contains the scaffold for use in our nanobody selection system. Containing two primary elements (lambda cl (codon optimized for Ecoli. and BH-GBP3) under the regulation of a lac-UV5 promoter. The two elements are separated with a XhoI cut site to allow for easy insertion of your naive or synthetic nanobody library.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 795
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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