Coding

Part:BBa_K2108001

Designed by: Maya de Vos   Group: iGEM16_NorthernBC   (2016-10-14)


YiaT-MCS

The E. coli membrane protein YiaT (truncated at R232) and fused with a multiple cloning site. The multiple cloning site is located on the extracellular side of the membrane and is on a long flexible arm of the C-terminal end of YiaT. This system allows for the expression of any protein to the outside of E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 641
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 641
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 790
    Illegal XhoI site found at 784
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 641
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 641
    Illegal NgoMIV site found at 325
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None