Plasmid_Backbone
Part:BBa_K2100081
Designed by: Archis R. Bhandarkar Group: iGEM16_MIT (2016-10-21)
pEXPR Backbone
This is the plasmid backbone of an expression vector after performing an LR reaction in Gateway cloning assembly. It contains attB and attP sites that are the result of the recombination of attL and attR. The selection marker for this expression vector is ampicillin.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 41 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 41 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 41
Illegal NgoMIV site found at 604 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1581
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Categories
Parameters
None |