Plasmid_Backbone

Part:BBa_K2100081

Designed by: Archis R. Bhandarkar   Group: iGEM16_MIT   (2016-10-21)


pEXPR Backbone

This is the plasmid backbone of an expression vector after performing an LR reaction in Gateway cloning assembly. It contains attB and attP sites that are the result of the recombination of attL and attR. The selection marker for this expression vector is ampicillin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 41
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 41
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 41
    Illegal NgoMIV site found at 604
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1581


[edit]
Categories
Parameters
None