DNA

Part:BBa_K2100080

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-19)


pEXPR hEF1a: Gal4 - VP16

This is a constitutive mammalian promoter with Gal4-VP16. In experiments involving repressible promoters, this part is often necessary because the repressible promoters need to be activated by Gal4. When Gal4-VP16 is constitutively expressed, the repressible promoters will express the downstream genes unless their respective repressors are present to inhibit transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal EcoRI site found at 1949
    Illegal PstI site found at 337
    Illegal PstI site found at 842
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal EcoRI site found at 1949
    Illegal PstI site found at 337
    Illegal PstI site found at 842
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal EcoRI site found at 1949
    Illegal BglII site found at 591
    Illegal BamHI site found at 1197
    Illegal BamHI site found at 1227
    Illegal XhoI site found at 990
    Illegal XhoI site found at 1703
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal EcoRI site found at 1949
    Illegal PstI site found at 337
    Illegal PstI site found at 842
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal EcoRI site found at 1949
    Illegal PstI site found at 337
    Illegal PstI site found at 842
    Illegal NgoMIV site found at 725
    Illegal AgeI site found at 103
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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