DNA

Part:BBa_K2100079

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-19)


pEXPR TRE: tagBFP

This part is a blue fluorescent protein under Dox-inducible promoter TRE. When testing a gene of interest under the TRE promoter, such as a repressor, this part can be used in conjunction to give a readout on how much the gene of interest is being expressed. Since the gene of interest and tagBFP are under the same promoter in the same conditions, their expression levels will be the same, so the amount of blue fluorescence, which is easily observable, can be used to determine the amount of expression of the gene of interest.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal BamHI site found at 339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None