DNA

Part:BBa_K2100078

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-19)


pEXPR EGSH - 4kturn: mKate

This part contains 4 k-turns to repress expression of red fluorescent protein mKate. This is the final expression vector created from a LR between pENTR EGSH 4xk-turn and pENTR mKate. mKate expression is suppressed by an inducible promoter, pEGSH (induced with Ponasterone A - PonA), and a RNA-based repressing system L7Ae/k-turn. This expression vector reduces the leaky expression (when the system is not induced with PonA) of mKate with the L7Ae/k-turn system.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1265
    Illegal XbaI site found at 2225
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1265
    Illegal NheI site found at 810
    Illegal NheI site found at 1076
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
    Illegal NotI site found at 2239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1265
    Illegal BglII site found at 1388
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1265
    Illegal XbaI site found at 2225
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1265
    Illegal XbaI site found at 2225
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4096
    Illegal SapI.rc site found at 687
    Illegal SapI.rc site found at 3478


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Categories
Parameters
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