DNA

Part:BBa_K2100077

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-19)


pEXPR EGSH - 4kturn: TP901

This part contains 4 k-turns to repress expression of serine integrase TP901.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 3863
    Illegal EcoRI site found at 4966
    Illegal XbaI site found at 2225
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 3863
    Illegal EcoRI site found at 4966
    Illegal NheI site found at 810
    Illegal NheI site found at 1076
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
    Illegal NotI site found at 2239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 3863
    Illegal EcoRI site found at 4966
    Illegal BglII site found at 1388
    Illegal BglII site found at 4746
    Illegal BamHI site found at 3450
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 3863
    Illegal EcoRI site found at 4966
    Illegal XbaI site found at 2225
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 3863
    Illegal EcoRI site found at 4966
    Illegal XbaI site found at 2225
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
    Illegal NgoMIV site found at 3614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 687


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Categories
Parameters
None