DNA

Part:BBa_K2100073

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-19)


pEXPR EGSH - 2kturn: mKate

This part contains 2 k-turns to repress expression of red fluorescent protein mKate. This is the final expression vector created from a LR between pENTR EGSH 2xk-turn and pENTR mKate. mKate expression is suppressed by an inducible promoter, pEGSH (induced with Ponasterone A - PonA), and a RNA-based repressing system L7Ae/k-turn. This expression vector reduces the leaky expression (when the system is not induced with PonA) of mKate with the L7Ae/k-turn system.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1287
    Illegal XbaI site found at 2193
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1287
    Illegal NheI site found at 832
    Illegal NheI site found at 1098
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
    Illegal NotI site found at 2207
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1287
    Illegal BglII site found at 1410
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1287
    Illegal XbaI site found at 2193
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1287
    Illegal XbaI site found at 2193
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4042
    Illegal SapI.rc site found at 709
    Illegal SapI.rc site found at 3424


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Categories
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