Part:BBa_K2100067

pEXPR hEF1a: VgEcR

This part contains constitutive mammalian promoter hEF1a and EGSH transactivator VgEcR. It produces a protein that activates the EGSH promoter upon binding small molecule PonA.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1210
  Illegal XbaI site found at 1517
  Illegal PstI site found at 337
  Illegal PstI site found at 842
  Illegal PstI site found at 3498
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1210
  Illegal NheI site found at 3317
  Illegal PstI site found at 337
  Illegal PstI site found at 842
  Illegal PstI site found at 3498
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1210
  Illegal BglII site found at 591
  Illegal BglII site found at 2811
  Illegal BamHI site found at 1197
  Illegal BamHI site found at 1712
  Illegal BamHI site found at 4188
  Illegal BamHI site found at 4341
  Illegal BamHI site found at 4455
  Illegal XhoI site found at 990
  Illegal XhoI site found at 2804
  Illegal XhoI site found at 2885
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1210
  Illegal XbaI site found at 1517
  Illegal PstI site found at 337
  Illegal PstI site found at 842
  Illegal PstI site found at 3498
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1210
  Illegal XbaI site found at 1517
  Illegal PstI site found at 337
  Illegal PstI site found at 842
  Illegal PstI site found at 3498
  Illegal NgoMIV site found at 725
  Illegal NgoMIV site found at 2933
  Illegal NgoMIV site found at 3280
  Illegal NgoMIV site found at 4753
  Illegal AgeI site found at 103
  Illegal AgeI site found at 4041
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 4226
  Illegal SapI.rc site found at 1615
  Illegal SapI.rc site found at 1747