DNA

Part:BBa_K2100061

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-19)


pENTR hEF1a-SV40

The pENTR hEF1a-Sv40 is an entry vector containing constitutive mammalian promoter human elongation factor 1 alpha (hEF1a) and transcriptional stop signal SV40. This part was designed to be used to prevent transcription of the gene that follows it. However, our experimental results show that this part did not successfully stop transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 29
    Illegal EcoRI site found at 1233
    Illegal XbaI site found at 1262
    Illegal PstI site found at 360
    Illegal PstI site found at 865
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 29
    Illegal EcoRI site found at 1233
    Illegal PstI site found at 360
    Illegal PstI site found at 865
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 29
    Illegal EcoRI site found at 1233
    Illegal BglII site found at 614
    Illegal BamHI site found at 1220
    Illegal XhoI site found at 1013
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 29
    Illegal EcoRI site found at 1233
    Illegal XbaI site found at 1262
    Illegal PstI site found at 360
    Illegal PstI site found at 865
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 29
    Illegal EcoRI site found at 1233
    Illegal XbaI site found at 1262
    Illegal PstI site found at 360
    Illegal PstI site found at 865
    Illegal NgoMIV site found at 748
    Illegal AgeI site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None