DNA
Part:BBa_K2100061
Designed by: Colleen Foley Group: iGEM16_MIT (2016-10-19)
pENTR hEF1a-SV40
The pENTR hEF1a-Sv40 is an entry vector containing constitutive mammalian promoter human elongation factor 1 alpha (hEF1a) and transcriptional stop signal SV40. This part was designed to be used to prevent transcription of the gene that follows it. However, our experimental results show that this part did not successfully stop transcription.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 29
Illegal EcoRI site found at 1233
Illegal XbaI site found at 1262
Illegal PstI site found at 360
Illegal PstI site found at 865 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 29
Illegal EcoRI site found at 1233
Illegal PstI site found at 360
Illegal PstI site found at 865 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 29
Illegal EcoRI site found at 1233
Illegal BglII site found at 614
Illegal BamHI site found at 1220
Illegal XhoI site found at 1013 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 29
Illegal EcoRI site found at 1233
Illegal XbaI site found at 1262
Illegal PstI site found at 360
Illegal PstI site found at 865 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 29
Illegal EcoRI site found at 1233
Illegal XbaI site found at 1262
Illegal PstI site found at 360
Illegal PstI site found at 865
Illegal NgoMIV site found at 748
Illegal AgeI site found at 126 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |