DNA

Part:BBa_K2100060

Designed by: Julia Goupil   Group: iGEM16_MIT   (2016-10-19)


Sensor 21-5p

This part contains two main transcriptional units. One encoding hef1a-mKate adjacent to 4 miRNA target sites. The other encodes hef1a-BFP. mKate production signifies the amount of repression occurring due to miRNA activity. BFP can be used as transfection marker.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 771
    Illegal EcoRI site found at 1424
    Illegal EcoRI site found at 2653
    Illegal EcoRI site found at 3449
    Illegal EcoRI site found at 7162
    Illegal EcoRI site found at 7790
    Illegal EcoRI site found at 8342
    Illegal XbaI site found at 8348
    Illegal SpeI site found at 5287
    Illegal PstI site found at 847
    Illegal PstI site found at 1130
    Illegal PstI site found at 1779
    Illegal PstI site found at 2284
    Illegal PstI site found at 3986
    Illegal PstI site found at 8327
    Illegal PstI site found at 8638
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 771
    Illegal EcoRI site found at 1424
    Illegal EcoRI site found at 2653
    Illegal EcoRI site found at 3449
    Illegal EcoRI site found at 7162
    Illegal EcoRI site found at 7790
    Illegal EcoRI site found at 8342
    Illegal SpeI site found at 5287
    Illegal PstI site found at 847
    Illegal PstI site found at 1130
    Illegal PstI site found at 1779
    Illegal PstI site found at 2284
    Illegal PstI site found at 3986
    Illegal PstI site found at 8327
    Illegal PstI site found at 8638
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 771
    Illegal EcoRI site found at 1424
    Illegal EcoRI site found at 2653
    Illegal EcoRI site found at 3449
    Illegal EcoRI site found at 7162
    Illegal EcoRI site found at 7790
    Illegal EcoRI site found at 8342
    Illegal BglII site found at 194
    Illegal BglII site found at 2033
    Illegal BglII site found at 3529
    Illegal BglII site found at 7870
    Illegal BglII site found at 9602
    Illegal BamHI site found at 10256
    Illegal XhoI site found at 729
    Illegal XhoI site found at 2432
    Illegal XhoI site found at 7118
    Illegal XhoI site found at 10001
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 771
    Illegal EcoRI site found at 1424
    Illegal EcoRI site found at 2653
    Illegal EcoRI site found at 3449
    Illegal EcoRI site found at 7162
    Illegal EcoRI site found at 7790
    Illegal EcoRI site found at 8342
    Illegal XbaI site found at 8348
    Illegal SpeI site found at 5287
    Illegal PstI site found at 847
    Illegal PstI site found at 1130
    Illegal PstI site found at 1779
    Illegal PstI site found at 2284
    Illegal PstI site found at 3986
    Illegal PstI site found at 8327
    Illegal PstI site found at 8638
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 771
    Illegal EcoRI site found at 1424
    Illegal EcoRI site found at 2653
    Illegal EcoRI site found at 3449
    Illegal EcoRI site found at 7162
    Illegal EcoRI site found at 7790
    Illegal EcoRI site found at 8342
    Illegal XbaI site found at 8348
    Illegal SpeI site found at 5287
    Illegal PstI site found at 847
    Illegal PstI site found at 1130
    Illegal PstI site found at 1779
    Illegal PstI site found at 2284
    Illegal PstI site found at 3986
    Illegal PstI site found at 8327
    Illegal PstI site found at 8638
    Illegal NgoMIV site found at 932
    Illegal NgoMIV site found at 1215
    Illegal NgoMIV site found at 2167
    Illegal NgoMIV site found at 8553
    Illegal NgoMIV site found at 8834
    Illegal NgoMIV site found at 9736
    Illegal AgeI site found at 1545
    Illegal AgeI site found at 8682
    Illegal AgeI site found at 9114
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7141
    Illegal BsaI site found at 11028
    Illegal BsaI.rc site found at 106


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Categories
Parameters
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