Generator

Part:BBa_K210006

Designed by: Makoto Kashima   Group: iGEM09_Kyoto   (2009-10-15)


EGFP::PEST sequence generator yeast-optimized

pTEF2(BBa_K165037)+kozak+EGFP+SV40 nuclear localization sequence + ADH1 terminator(BBa_K165018)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1247
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1064


[edit]
Categories
Parameters
None