DNA

Part:BBa_K2100053

Designed by: Wangui Mbuguiro   Group: iGEM16_MIT   (2016-10-19)


Sensor 142-5p

This part contains two main transcriptional units. One encoding hef1a-mKate adjacent to 4 miRNA target sites. The other encodes hef1a-BFP. mKate production signifies the amount of repression occurring due to miRNA activity. BFP can be used as transfection marker.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 767
    Illegal EcoRI site found at 1420
    Illegal EcoRI site found at 2649
    Illegal EcoRI site found at 3445
    Illegal EcoRI site found at 7158
    Illegal EcoRI site found at 7786
    Illegal EcoRI site found at 8338
    Illegal XbaI site found at 8344
    Illegal SpeI site found at 5283
    Illegal PstI site found at 843
    Illegal PstI site found at 1126
    Illegal PstI site found at 1775
    Illegal PstI site found at 2280
    Illegal PstI site found at 3982
    Illegal PstI site found at 8323
    Illegal PstI site found at 8634
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 767
    Illegal EcoRI site found at 1420
    Illegal EcoRI site found at 2649
    Illegal EcoRI site found at 3445
    Illegal EcoRI site found at 7158
    Illegal EcoRI site found at 7786
    Illegal EcoRI site found at 8338
    Illegal SpeI site found at 5283
    Illegal PstI site found at 843
    Illegal PstI site found at 1126
    Illegal PstI site found at 1775
    Illegal PstI site found at 2280
    Illegal PstI site found at 3982
    Illegal PstI site found at 8323
    Illegal PstI site found at 8634
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 767
    Illegal EcoRI site found at 1420
    Illegal EcoRI site found at 2649
    Illegal EcoRI site found at 3445
    Illegal EcoRI site found at 7158
    Illegal EcoRI site found at 7786
    Illegal EcoRI site found at 8338
    Illegal BglII site found at 190
    Illegal BglII site found at 2029
    Illegal BglII site found at 3525
    Illegal BglII site found at 7866
    Illegal BglII site found at 9598
    Illegal BamHI site found at 10252
    Illegal XhoI site found at 725
    Illegal XhoI site found at 2428
    Illegal XhoI site found at 7114
    Illegal XhoI site found at 9997
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 767
    Illegal EcoRI site found at 1420
    Illegal EcoRI site found at 2649
    Illegal EcoRI site found at 3445
    Illegal EcoRI site found at 7158
    Illegal EcoRI site found at 7786
    Illegal EcoRI site found at 8338
    Illegal XbaI site found at 8344
    Illegal SpeI site found at 5283
    Illegal PstI site found at 843
    Illegal PstI site found at 1126
    Illegal PstI site found at 1775
    Illegal PstI site found at 2280
    Illegal PstI site found at 3982
    Illegal PstI site found at 8323
    Illegal PstI site found at 8634
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 767
    Illegal EcoRI site found at 1420
    Illegal EcoRI site found at 2649
    Illegal EcoRI site found at 3445
    Illegal EcoRI site found at 7158
    Illegal EcoRI site found at 7786
    Illegal EcoRI site found at 8338
    Illegal XbaI site found at 8344
    Illegal SpeI site found at 5283
    Illegal PstI site found at 843
    Illegal PstI site found at 1126
    Illegal PstI site found at 1775
    Illegal PstI site found at 2280
    Illegal PstI site found at 3982
    Illegal PstI site found at 8323
    Illegal PstI site found at 8634
    Illegal NgoMIV site found at 928
    Illegal NgoMIV site found at 1211
    Illegal NgoMIV site found at 2163
    Illegal NgoMIV site found at 8549
    Illegal NgoMIV site found at 8830
    Illegal NgoMIV site found at 9732
    Illegal AgeI site found at 1541
    Illegal AgeI site found at 8678
    Illegal AgeI site found at 9110
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7137
    Illegal BsaI site found at 11024
    Illegal BsaI.rc site found at 102


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Parameters
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