Deleted
Sample Discontinued
Experience: None
Not Used
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Part:BBa_K2100046

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-17)


pEXPR pBM3R1:mKate

This construct is the result of a gateway combining a BM3R1 repressible promoter upstream of the mKate gene, a red fluorescent protein. pBM3R1 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The BM3R1 repressor inhibits the activator and transcription machinery from transcribing the mKate gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 242
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 242
    Illegal NheI site found at 115
    Illegal NotI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 242
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 108
    Illegal BsaI site found at 235
    Illegal BsaI.rc site found at 906
    Illegal SapI.rc site found at 288


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