DNA
Part:BBa_K2100045
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-10-17)
pEXPR pTALER14:mKate
This construct is the result of a gateway combining a TALER14 repressible promoter upstream of the mKate gene, a red fluorescent protein. pTALER14 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The TALER14 repressor inhibits the activator and transcription machinery from transcribing the mKate gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal BamHI site found at 303
Illegal XhoI site found at 246 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 318
Illegal BsaI.rc site found at 999
Illegal SapI.rc site found at 381
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Categories
Parameters
None |