DNA

Part:BBa_K2100045

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-17)


pEXPR pTALER14:mKate

This construct is the result of a gateway combining a TALER14 repressible promoter upstream of the mKate gene, a red fluorescent protein. pTALER14 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The TALER14 repressor inhibits the activator and transcription machinery from transcribing the mKate gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal XbaI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal BamHI site found at 303
    Illegal XhoI site found at 246
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal XbaI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal XbaI site found at 157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 318
    Illegal BsaI.rc site found at 999
    Illegal SapI.rc site found at 381


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