DNA
Part:BBa_K2100039
Designed by: Wangui Mbuguiro Group: iGEM16_MIT (2016-10-17)
pEXPR pBM3R1 - eYFP
This construct is the result of a gateway combining a BM3R1 repressible promoter upstream of the eYFP gene, a yellow fluorescent protein. pBM3R1 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The BM3R1 repressor inhibits the activator and transcription machinery from transcribing the eYFP gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 242
Illegal PstI site found at 481 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 242
Illegal NheI site found at 115
Illegal PstI site found at 481
Illegal NotI site found at 4 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 242
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 242
Illegal PstI site found at 481 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 242
Illegal PstI site found at 481
Illegal AgeI site found at 264 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 108
Illegal BsaI site found at 235
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Categories
Parameters
None |