DNA
Part:BBa_K2100038
Designed by: Wangui Mbuguiro Group: iGEM16_MIT (2016-10-17)
pEXPR TRE - TALER21
This construct is an expression vector containing the TRE promoter upstream of the TALER21 gene. The promoter is structured as six tetO sites upstream of a minimal CMV promoter. The induction of doxycycline activates a tetracycline transactivator protein which in turn binds to the tetO sites to initiate transcription of the TALER21 repressor. TALER14 is an engineered repressor from TAL effectors, amino acids which have corresponding DNA target sites. When transcribed, TALER21 represses the DNA sequences found on the pTALER21 promoter with the help of the corepressor VP16-Gal4.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 344
Illegal EcoRI site found at 520
Illegal XbaI site found at 30
Illegal XbaI site found at 495 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 344
Illegal EcoRI site found at 520 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 344
Illegal EcoRI site found at 520
Illegal BamHI site found at 649
Illegal XhoI site found at 586 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 344
Illegal EcoRI site found at 520
Illegal XbaI site found at 30
Illegal XbaI site found at 495 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 344
Illegal EcoRI site found at 520
Illegal XbaI site found at 30
Illegal XbaI site found at 495 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 664
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Categories
Parameters
None |