DNA

Part:BBa_K2100023

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-14)


pENTR pTALER21

The entry vector contains a promoter repressible by the TAL21 repressor. pTAL21 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. (Li et al., 2015) [1] A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The TAL21 repressor inhibits the activator and transcription machinery from transcribing the gene downstream of pTAL21.

[1] Li, Y., Jiang, Y., Chen, H., Liao, W., Li, Z., Weiss, R., & Xie, Z. (2015). Modular construction of mammalian gene circuits using TALE transcriptional repressors. Nature Chemical Biology, 11(3), 207–213. http://doi.org/10.1038/nchembio.1736 Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 182
    Illegal BamHI site found at 311
    Illegal XhoI site found at 248
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 326
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 157


[edit]
Categories
//awards/part_collection/2016
Parameters
None