DNA

Part:BBa_K2100020

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-14)


pENTR GAL4-VP16

Gal4-VP16 is a transactivator that contains Gal4, a DNA binding protein derived from yeast, fused to VP16, an activation domain derived from the herpes simplex virus. Gal4-VP16 binds to Gal4 sites in a promoter and recruits the eukarytoic transcriptional machinery through interactions mediated by VP16.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 723
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 723
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 723
    Illegal BamHI site found at 1
    Illegal XhoI site found at 477
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 723
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 723
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2016
Parameters
None